![[Next-Generation Sequencing Analysis Case] Analysis of Live Bacterial Microbial Community Structure](https://image.mono.ipros.com/public/product/image/2a0/2000603371/IPROS20415915303374144225.png?w=280&h=280)
[Next-Generation Sequencing Analysis Case] Analysis of Live Bacterial Microbial Community Structure
Introducing a case that demonstrates the method using EMA can analyze live microbial communities! *Case study collection available.
We will introduce community structure analysis using the selective membrane permeable dye EMA (in-house data). For environmental samples dominated by E. coli, we prepared samples that were heat-treated and sterilized, as well as untreated samples, and performed EMA treatment. Subsequently, DNA extraction was conducted for each sample, followed by 16S rRNA gene amplicon analysis. As a result, in the community structure analysis, E. coli, which had become dominant, was hardly detected in the heat-treated samples due to its death. Additionally, in the related links, we also introduce community structure analysis using reverse transcription cDNA from RNA (in-house data), so please take a look. 【Workflow】 ■ Prepare environmental samples (heat-treated, untreated) ■ EMA treatment ■ Light irradiation ■ DNA extraction ■ Creation of adapter library via EMA-PCR *For more details, please refer to the PDF document or feel free to contact us.
- Company：ファスマック
- Price：Other
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